proteome profiler mouse adipokine array kit  (Bio-Techne corporation)

Journal: Bioactive Materials

Article Title: Amphiphilic cytokine traps remodel marrow adipose tissue for hematopoietic microenvironment amelioration

doi: 10.1016/j.bioactmat.2024.08.032

Figure Lengend Snippet: Changes, especially browning, in the expression of proteins about adipocyte metabolism in marrow induced by AC-gelatin composites. (A) Fluorescence colocalization imaging of the UCP1 + adipocytes through represented by UCP1 and Plin (white triangles); all scale bars are 200 μm. (B) MAT browning promotion by AC-gelatin at 5 weeks in statistics of Fig. A. (C) Protein expression tests of osteo-organoid and bone marrow by Western blotting; β-tubulin, a protein expressed stably in adipocytes, was used as the internal reference. (D) Protein expression tests of whole marrow by protein microarray (Proteome Profiler Mouse Adipokine Array Kit, R&D Systems) and the quantified data (E). Error bars: mean ± SD; n = 2 (Fig. E, number of wells) or 3 (the others, number of mice); * p < 0.05, ** p < 0.01, **** p < 0.0001, two-way ANOVA.

Article Snippet: Changes, especially browning, in the expression of proteins about adipocyte metabolism in marrow induced by AC-gelatin composites. (A) Fluorescence colocalization imaging of the UCP1 + adipocytes through represented by UCP1 and Plin (white triangles); all scale bars are 200 μm. (B) MAT browning promotion by AC-gelatin at 5 weeks in statistics of Fig. A. (C) Protein expression tests of osteo-organoid and bone marrow by Western blotting; β-tubulin, a protein expressed stably in adipocytes, was used as the internal reference. (D) Protein expression tests of whole marrow by protein microarray (Proteome Profiler Mouse Adipokine Array Kit, R&D Systems) and the quantified data (E).

Techniques: Expressing, Fluorescence, Imaging, Western Blot, Stable Transfection, Microarray